本人主要从事哺乳动物卵泡发育的分子调控机理研究。研究侧重点包括:1. 引起卵泡氧化损伤的信号通路与效应分子;2. 卵泡液因子对卵泡氧化损伤的调控机制;3. 抗氧化剂对动物繁殖性能的改善;4.卵泡低氧环境对卵泡发育的影响及机制;5.卵泡发育过程中卵母细胞与颗粒细胞互作机制;6.卵泡微环境影响卵泡发育的表观遗传机理。在国家重点研发项目、国家自然科学基金、江苏省自然科学基金等项目支持下,已在Autophagy(IF5=16.142)、Redox biology(IF5=11.271)、Development、Journal of Biological Chemistry、Faseb Journal、Endocrinology、Biology of Reproduction、Cell Death & disease、Reproduction、Theriogenology等领域前20%或影响因子>5 的刊物发表第一/通讯作者论文多篇。研究工作得到国内外同行的肯定,已被多本英文著作如Endocrine Cells: Advances in Research and Application(第21-22 页,2013年出版,出版社Scholarly EditionsTM)、World Clinics: Dermatology-Acne(第72 页,2013 年出版,出版社Jaypee Brothers Medical Pub)、International Review of Cell and Molecular Biology(第33 页,2018 年出版,出版社Academic Press)、IntechOpen Book Series Physiology, Volume 2: Endoplasmic Reticulum(第80-81页,2019 年出版,出版社IntechOpen)收录并介绍。相关研究成果对于认清卵泡发育/闭锁转换规律、丰富和完善繁殖学理论、提高动物繁殖性能以及治疗人类排卵障碍相关疾病都具有重要意义。 一、10篇代表性论著 (1)Shen M, Jiang Y, Guan Z, Cao Y, Li L, Liu H, Sun SC. (2017) Protective mechanism of FSH against oxidative damage in mouse ovarian granulosa cells by repressing autophagy. Autophagy. 13(8): 1364~1385, 第一兼通讯作者 (2)Shen M, Cao Y, Jiang Y, Wei Y, Liu H. (2018) Melatonin protects mouse granulosa cells against oxidative damage by inhibiting FOXO1-mediated autophagy: Implication of an antioxidation-independent mechanism. Redox Biology. 18:138-157 (3)Li C, Liu Z, Wu G, Zang Z, Zhang JQ, Li X, Tao J, Shen M, Liu H. (2021) FOXO1 mediates hypoxia-induced G0/G1 arrest in ovarian somatic granulosa cells by activating the TP53INP1-p53-CDKN1A pathway. Development. 148(14):dev199453,通讯作者 (4)Li C, Meng X, Liu S, Li W, Zhang X, Zhou J, Yao W, Dong C, Liu Z, Zhou J, Li J, Tao J, Wu W, Shen M, Liu H. (2020) Oocytes and hypoxanthine orchestrate the G2-M switch mechanism in ovarian granulosa cells. Development. 147(13):dev184838,通讯作者 (5)Li C, Zhou J, Liu Z, Zhou J, Yao W, Tao J, Shen M, Liu H. (2020) FSH prevents porcine granulosa cells from hypoxia-induced apoptosis via activating mitophagy through the HIF-1α-PINK1-Parkin pathway. FASEB J. 34(3):3631-3645,通讯作者 (6)Wu G, Li C, Tao J, Liu Z, Li X, Zang Z, Fu C, Wei J, Yang Y, Zhu Q, Zhang JQ, Shen M, Liu H. (2022) FSH mediates estradiol synthesis in hypoxic granulosa cells by activating glycolytic metabolism through the HIF-1α-AMPK-GLUT1 signaling pathway. J Biol Chem. 298(5):101830,通讯作者 (7)Shen, M., Lin, F., Zhang, J., Tang, Y., Chen, W. K., and Liu, H. (2012) Involvement of the upregulated FoxO1 expression in follicular granulosa cell apoptosis induced by oxidative stress. J Biol Chem. 287(31):25727-40 (8)Li C, Liu Z, Li W, Zhang L, Zhou J, Sun M, Zhou J, Yao W, Zhang X, Wang H, Tao J, Shen M, Liu H. (2020) The FSH-HIF-1α-VEGF Pathway Is Critical for Ovulation and Oocyte Health but Not Necessary for Follicular Growth in Mice. Endocrinology. 161(4):bqaa038,通讯作者 (9)Li C, Liu Z, Zhou J, Meng X, Liu S, Li W, Zhang X, Zhou J, Yao W, Dong C, Cao Y, Li R, Chen B, Jiang A, Jiang Y, Ning C, Zhao F, Wei Y, Sun SC, Tao J, Wu W, Shen M, Liu H. (2020) Insulin-like growth factor-I prevents hypoxia-inducible factor-1 alpha-dependent G1/S arrest by activating cyclin E/cyclin-dependent kinase2 via the phoshatidylinositol-3 kinase/AKT/forkhead box O1/Cdkn1b pathway in porcine granulosa cells. Biol Reprod. 102(1):116-132,通讯作者 (10)Shen M, Liu Z, Li B, Teng Y, Zhang J, Tang Y, Sun SC, Liu H. (2014) Involvement of FoxO1 in the effects of follicle-stimulating hormone on inhibition of apoptosis in mouse granulosa cells. Cell Death Dis. 5:e1475 二、其他论著 (1)Zhang X, Wei Y, Li X, Li C, Zhang L, Liu Z, Cao Y, Li W, Zhang X, Zhang J, Shen M, Liu H. (2022) The Corticosterone-Glucocorticoid Receptor-AP1/CREB Axis Inhibits the Luteinizing Hormone Receptor Expression in Mouse Granulosa Cells. Int J Mol Sci. 23(20):12454,通讯作者 (2)Zhang JQ, Lykke-Hartmann K, Shen M. (2021) Editorial: Involvement of Autophagy in Determining the Developmental Fate of Ovarian Follicles. Front Cell Dev Biol. 9:814135,通讯作者 (3)Liu Z, Li C, Wu G, Li W, Zhang X, Zhou J, Zhang L, Tao J, Shen M, Liu H. (2020) Involvement of JNK/FOXO1 pathway in apoptosis induced by severe hypoxia in porcine granulosa cells. Theriogenology. 154:120-127,通讯作者 (4)Cao Y, Shen M, Jiang Y, Sun SC, Liu HL. (2018) Melatonin reduces oxidative damage in mouse granulosa cells via restraining JNK-dependent autophagy. Reproduction. 155(3): 307~319, 共同一作兼通讯作者 (5)Wei Y, Li W, Meng X, Zhang L, Shen M, Liu H. (2019) Corticosterone Injection Impairs Follicular Development, Ovulation and Steroidogenesis Capacity in Mice Ovary. Animals (Basel). 9(12):1047,通讯作者 (6)魏甲园; 邾倩; 杨亚星; 申明. (2022) 氯化钴通过诱发DNA氧化损伤抑制猪卵泡颗粒细胞增殖的研究, 畜牧兽医学报, 53(09):2982~2992,通讯作者 (7)Shen M, Jiang Y, Guan Z, Cao Y, Sun SC, Liu H. (2016) FSH protects mouse granulosa cells from oxidative damage by repressing mitophagy. Sci Rep. 6: 38090~38090, 第一兼通讯作者 (8)Dai W, Liu K, Li R, Cao Y, Shen M, Tao J, Liu H. (2022) Trillin inhibits myoblast differentiation via increasing autophagy. Phytomedicine. 99:153962. (9)Zhang J, Ren Q, Chen J, Lv L, Wang J, Shen M, Xing B, Wang X. (2022) Downregulation of miR-192 Alleviates Oxidative Stress-Induced Porcine Granulosa Cell Injury by Directly Targeting Acvr2a. Cells. 11. (10)Tao J, Zhang L, Zhang X, Chen Y, Chen Q, Shen M, Liu H, Deng S. (2021) Effect of Exogenous Melatonin on the Development of Mice Ovarian Follicles and Follicular Angiogenesis. Int J Mol Sci. 22. (11)Jiang Y, Shen M, Chen Y, Wei Y, Tao J, Liu H. (2021) Melatonin Represses Mitophagy to Protect Mouse Granulosa Cells from Oxidative Damage. Biomolecules. 11. (12)Cao Y, Li R, Shen M, Li C, Zou Y, Jiang Q, Liu S, Lu C, Li H, Liu H, et al. (2021) DDRGK1, a crucial player of ufmylation system, is indispensable for autophagic degradation by regulating lysosomal function. Cell Death Dis. 12:416. (13)Daniel J. Klionsky, Amal Kamal Abdel-Aziz, ..., Ming Shen, et al. (2021) Guidelines for the Use and Interpretation of Assays for Monitoring Autophagy (4th edition). Autophagy, Feb 8;1-382, (14)Tao JL, Zhang X, Zhou JQ, Li CY, Yang MH, Liu ZJ, Zhang LL, Deng SL, Zhang L, Shen M, et al. (2021) Melatonin Alleviates Hypoxia-Induced Apoptosis of Granulosa Cells by Reducing ROS and Activating MTNR1B-PKA-Caspase8/9 Pathway. Antioxidants (Basel, Switzerland). 10. (15)Li R, Li B, Shen M, Cao Y, Zhang X, Li W, Tao J, Wu W, Liu H. (2020) LncRNA 2310043L19Rik inhibits differentiation and promotes proliferation of myoblast by sponging miR-125a-5p. Aging (Albany NY). 12:5625-5639. (16)Liu S; Shen M; Li C; Wei Y; Meng X; Li R; Cao Y; Wu W; Liu H. (2019) PKCδ contributes to oxidative stress-induced apoptosis in porcine ovarian granulosa cells via activating JNK. Theriogenology, Jun;131:89-95, IF5=2.316 (17)Zhao F; Wu W; Wei Q; Shen M; Li B; Jiang Y; Liu K; Liu H. (2018) Exogenous adrenocorticotropic hormone affects genome-wide DNA methylation and transcriptome of corpus luteum in sows, Faseb Journal, fj201801081RRR, IF5=5.421 (18)Li B; Weng Q; Liu Z; Shen M; Zhang J; Wu W; Liu H. (2017) Selection of antioxidants against ovarian oxidative stress in mouse model, J Biochem Mol Toxicol, 31(12) (19)Tang, Yiting; Pan, Bing; Zhou, Xin; Xiong, Kai; Gao, Qian; Huang, Lei; Xia, Ying; Shen, Ming; Yang, Shulin; Liu, Honglin; Tan, Tao; Ma, Jianjie; Xu, Xuehong; Mu, Yulian; Li, Kui. (2017) Wip1-dependent modulation of macrophage migration and phagocytosis, Redox Biology, 13:665-673, (20)李烈川,申明,宁彩波,管志强,蒋毅,吴望军,刘红林. (2016) 过氧化氢诱导猪卵巢颗粒细胞自噬及其对凋亡的影响, 南京农业大学学报, 39(5):814~818 (21)Huang, Xian-Ju; Shen, Ming; Wang, Lizhong; Yu, Fengxiang; Wu, Wangjun; Liu, Hong-Lin. (2015) Effects of tributyltin chloride on developing mouse oocytes and preimplantation embryos, Microscopy and Microanalysis, 21(2):358~367 (22)Zhang JQ; Xing BS; Zhu CC; Shen M; Yu FX; Hong-Lin Liu. (2015) Protective effect of proanthocyanidin against oxidative ovarian damage induced by 3-nitropropionic acid in mice, Genet Mol Res, 14(1):2484~2494 (23)Yiting Tang; Kai Xiong; Ming Shen; Yulian Mu; Kui Li; Honglin Liu. (2015) CCAAT-enhancer binding protein (C/EBP) beta regulates insulin-like growth factor (IGF) 1 expression in porcine liver during prenatal and postnatal development, Mol Cell Biochem, 401(1-2):209~218 (24)Liu, Ze-Qun; Shen, Ming; Wu, Wang-Jun; Li, Bo-Jiang; Weng, Qian-Nan; Li, Mei; Liu, Hong-Lin. (2015) Expression of PUMA in Follicular Granulosa Cells Regulated by FoxO1 Activation During Oxidative Stress, Reproductive Sciences, 22(6):696~705 (25)Yiting Tang; Lan Liu; Ming Shen; Kai Xiong; Lei Huang; Qian Gao; Jingliang Wei; Tianwen Wu; Shulin Yang; Honglin Liu; Yulian Mu; Kui Li. (2015) Wip1 knockout inhibits the proliferation and enhances the migration of bone marrow mesenchymal stem cells, Experimental Cell Research, 334(2):310~322 (26)Lin F; Fu YH; Han J; Shen M; Du CW; Li R; Ma XS; Liu HL. (2014) Changes in the expression of Fox O1 and death ligand genes during follicular atresia in porcine ovary, Genet Mol Res, 13(3): 6638-6645 (27)Zhang, Jia-Qing; Shen, Ming; Zhu, Cheng-Cheng; Yu, Feng-Xiang; Liu, Ze-Qun; Ally, Nazim; Sun, Shao-Chen; Li, Kui; Liu, Hong-Lin. (2014) 3-Nitropropionic acid induces ovarian oxidative stress and impairs follicle in mouse, Plos One, 9(2): e86589~e86589 (28)李诚瑜, 刘昭君, 申明, 李荣阳, 陶景丽, 吴望军, 刘红林. (2020) MSTN打靶猪的安全性分析, 农业技术与装备. 143-144+146 (29)张良良, 张轩, 周佳奇, 申明, 陶景丽, 刘红林. (2022) 褪黑素与卵泡刺激素对小鼠卵巢卵泡发育及其血管生成的影响, 畜牧与兽医. 54:1-8 (30)刘泽; 高峰; 周光宏; 申明. (2012) 牛蒡子苷对分化的小鼠C2C12细胞的影响, 营养学报, (04): 395~399 三、授权发明专利: 1. 刘红林,申明,吴望军 一种通过日粮添加原花青素提高母猪繁殖性能的方法,2022年4月8日,中国,ZL201811546492.9 2. 张家庆,李彦鹏,申明,施文创,王献伟,李冬冬,吕玲燕,苏格,卢清侠,孙浩,陈宝剑,李贺鹏,蒋凡,蒋立云 用于治疗猪卵巢囊肿的穿刺装置,2021年2月12日,中国,ZL202021694469.7 3. 张家庆,李彦鹏,王献伟,孙茂,申明,左连亭,陈宝剑,徐明增,吕玲燕,张诚民,蒋凡,朱红卫,卢清侠,张红剑 一种成年猪外科手术用固定装置,2021年2月12日,中国,ZL202021629992.1 4. 张家庆,李彦鹏,王献伟,胡言,蒋凡,孙茂,申明,施文创,陈宝剑,张毅,吕玲燕,李冬冬,卢清侠,蒋立云 一种提高母猪配种效率的模拟公猪,2021年2月12日,中国,ZL202021621887.3 四、国家计算机软件著作权登记证书: 1. 张家庆,吕玲燕,王献伟,申明,牛刚,高彬文 猪子宫膜炎定性辅助诊断系统V1.0,2022年12月06日,中国,2023SR0358390 |